Category: SciencePage 2 of 20

Generating a phylogeny of Polytrichum using hybrid baits

The current Next Gen Sequencing lab project at the Botanics involves looking at the phylogeny of Polytrichum section Polytrichum, using hybrid capture. The work will form part of…

Hybrid capture from degraded DNA: bead-cleaning away the adaptor peaks

After testing bead-to-sample ratios of 30:50, 35:50, 40:50, 50:50 and 60:50 on a Thermo Scientific™ GeneRuler™ 50 bp DNA Ladder, using Beckman Coulter AMPure XP beads, we focused…

Hybrid capture from degraded DNA: testing bead cleaning on a 50bp ladder

When we made our Begonia libraries, working with (in some cases) relatively small quantities of very degraded DNA, we should have diluted the adaptors. We didn’t. Consequently, we…

Rare Book Collection

Buchan Hepburn and McDouall East Africa and Angola Album 1904 – 1909

By Helen Bennett, Library & Archives Volunteer New light has been shed on the creation of Logan Garden by a previously unknown album of photographs and watercolours recently…

Rare Book Collection

Interleaving in the RBGE Collections, Part 1: The Flora of Forfarshire

By Hannah Swan Before publisher’s bindings were de rigueur, texts came in flimsy paper ‘wrappers’, leaving the permanent binding to the new owner. Because of this, many books from…

Hybrid capture from degraded DNA: quality metrics for duplicate extractions

For one of the taxa in our study set, Begonia scottii (living collection no. 20170076), we made a few replicate DNA extractions using Qiagen DNeasy plant mini-kits, and…

Meet the Valerian Family

“It’ll stink!  Tell me if you can’t stand it.  My cat adores it.”  Sweaty armpits, musty old rooms, smelly feet were all attempts at describing the smell of…

Hybrid capture from degraded DNA: bead cleaning degraded Begonia DNAs

The set of DNA extractions from one of our test plants (#8, Begonia stictopoda RBGE accession 20170115) contained rather low concentrations of DNA. We decided against preparing NGS…

An expedition to East Ross to rescue the rare Oxytropis halleri

Oxytropis halleri commonly known as Mountain Milk-vetch is a Nationally Rare plant confined only to Scotland.  The species can be found at two inland locations but most populations…

In memory of Gunner Andrew Ewing Calder (1884-1918)

Andrew Ewing Calder was born in Stockbridge, Edinburgh, on the 12th January 1884 to Robert Calder, a blacksmith, and his wife Sarah Jane, who was a tailoress. Calder…

How much liverwort do you need to get 50 μg of DNA?

There’s an exciting project, The 10KP (10,000 Plants) Genome Sequencing Project, that aims to sequence and characterize representative genomes from every major clade of embryophytes, green algae, and…

‘Have you seen the octopus?’

‘Hai visto il polpo?’ *Waves arms to impersonate octopus* ‘Its warm today isn’t it mate’ I said while flapping my arms back to him My current boss called…

Unusual fern Lepisorus thunbergianus

I spotted this unusual fern on a shipment that had come to Vannucci from Japan. The 8-20cm long plants were clinging to the trunk and underside of branches…

Protecting Potatoes: Diversity, Domestication and Darwin

Protecting Potatoes is a new plant display with interpretation for summer 2018 at the Botanics. It can be found in the Demonstration Garden and the Temperate Palm House,…

100th birthday for a Himalayan Wild Pear collected by George Forrest

On the 18th July 2018 we celebrate the 100th birthday of the Pyrus pashia tree growing on the Pyrus lawn.

British Council workshop on Valuing Andean Biodiversity

The dry and montane forests of the Andes are vital for the lives of tens of millions of people in western South America. Their socio-economic worth in cycling…

Crowd-sourcing finds new UK tree pest within two days!

To crowdsource information and tasks is an everyday part of the interconnected online world we live in. Today scientists see, and have grasped, the opportunity to get labour…

Disentangling the history of the Robert Brown specimens at RBGE

Robert Brown (1773-1858) Born in Montrose, Scotland in 1773, Robert Brown made his mark as a scientist in botany and palaeobotany. He is most famously known for his…

Upcoming Talks during the Big Botanics BioBlitz this Saturday

As part of our BioBlitz festivities, we are hosting four fabulous speakers to talk on a range of wildlife-related topics.   999 and counting… Recording Biodiversity at RBGE…

Chelsea Flower Show 2018

  This year was my first year at Chelsea. I was lucky enough to be able to work at Chelsea for Kevock garden plants. The display had a…