In memory of Corporal Henry McBeath (1878-1918)

Henry McBeath was from Rogart in Sutherland, born in November 1878 to James McBeath, a farm grieve (foreman) and his wife Johan from Rovie, a farm in Rogart…

Polytrichum baits: cutting cleaned mosses

Following on from the rather unpredictable results we obtained from fragmenting duplicate aliquots of CTAB-extracted Polytrichum DNA in the Bioruptor, Isuru cleaned aliquots of IK31 and IK53 using…

Hybrid capture from degraded DNA: the mysterious case of the vanishing libraries

When we’re working out a protocol or troubleshooting, we spend a lot of time quantifying small quantities of fluids, looking at DNA concentrations on the DeNovix, running tapes…

Polytrichum baits: Cutting mosses

We started our lab work on the Polytrichum hybrid baits project on the 1st of October, by normalising some CTAB-extracted DNA with 0.1X TE to 55 µL of…

Generating a phylogeny of Polytrichum using hybrid baits

The current Next Gen Sequencing lab project at the Botanics involves looking at the phylogeny of Polytrichum section Polytrichum, using hybrid capture. The work will form part of…

Hybrid capture from degraded DNA: bead-cleaning away the adaptor peaks

After testing bead-to-sample ratios of 30:50, 35:50, 40:50, 50:50 and 60:50 on a Thermo Scientific™ GeneRuler™ 50 bp DNA Ladder, using Beckman Coulter AMPure XP beads, we focused…

Hybrid capture from degraded DNA: testing bead cleaning on a 50bp ladder

When we made our Begonia libraries, working with (in some cases) relatively small quantities of very degraded DNA, we should have diluted the adaptors. We didn’t. Consequently, we…

Cast to mud

This is the season where worm casts appear on the surface of the lawn. If left and walked or worse, mown over, the squashing, flattening action will result…

Rare Book Collection

Buchan Hepburn and McDouall East Africa and Angola Album 1904 – 1909

By Helen Bennett, Library & Archives Volunteer New light has been shed on the creation of Logan Garden by a previously unknown album of photographs and watercolours recently…

Rare Book Collection

Interleaving in the RBGE Collections, Part 1: The Flora of Forfarshire

Before publisher’s bindings were de rigueur, texts came in flimsy paper ‘wrappers’, leaving the permanent binding to the new owner. Because of this, many books from the 19th century…

Clematis terniflora

Seed heads

The seed pods of Clematis terniflora have split open and are releasing their seed. The plumose styles appear as a mass of white feathers covering the tangled growth….

Hybrid capture from degraded DNA: quality metrics for duplicate extractions

For one of the taxa in our study set, Begonia scottii (living collection no. 20170076), we made a few replicate DNA extractions using Qiagen DNeasy plant mini-kits, and…

Meet the Valerian Family

“It’ll stink!  Tell me if you can’t stand it.  My cat adores it.”  Sweaty armpits, musty old rooms, smelly feet were all attempts at describing the smell of…

Natural Selection Exhibition at Inverleith House

Inverleith house has produced many wonderful exhibitions. The Lost Words exhibition being a great success with the many visitors that attended. The newest addition to Inverleith house is…

Hybrid capture from degraded DNA: bead cleaning degraded Begonia DNAs

The set of DNA extractions from one of our test plants (#8, Begonia stictopoda RBGE accession 20170115) contained rather low concentrations of DNA. We decided against preparing NGS…

An expedition to East Ross to rescue the rare Oxytropis halleri

Oxytropis halleri commonly known as Mountain Milk-vetch is a Nationally Rare plant confined only to Scotland.  The species can be found at two inland locations but most populations…

A perennial daisy

The space around one of the alpine troughs is awash with a multitude of small daisy like flowers. From minute green button buds white ray florets quickly turn…

Castanea sativa

But will there be Chestnuts to roast?

The iconic Sweet Chestnut on the NW corner of the Rock Garden has sustained damage to the canopy over the years. The westerly storm force winds have ripped…

Polylepis australis – Tree of the Year Finalist 2018 -Please vote!

Help Make The Filo Pastry Tree at Logan Botanic Garden Woodland Trust Scotland’s Tree of the Year. Please help us to win this award by voting for the…

Digging for Potato Treasure

The RBGE Harvest Festival is always an incredible display of produce from gardens all over the City, but in 2018 the Botanics had an unusual contribution to the…