Category: Science Page 1 of 19

Local network, social media and a new generic ginger record for Nepal

The torch ginger genus, Etlingera, is distributed from India in the western through SE Asia to Australia and Fiji in the east. Botanical collections made in the 19th…

WeDigBio logo

WeDigBio at the Botanics

There’s a first time for everything and last week the RBGE herbarium digitisation team hosted its first WeDigBio onsite transcription event! Worldwide Engagement for Digitising Biocollections, WeDigBio, is…

In memory of Corporal Henry McBeath (1878-1918)

Henry McBeath was from Rogart in Sutherland, born in November 1878 to James McBeath, a farm grieve (foreman) and his wife Johan from Rovie, a farm in Rogart…

Polytrichum baits: cutting cleaned mosses

Following on from the rather unpredictable results we obtained from fragmenting duplicate aliquots of CTAB-extracted Polytrichum DNA in the Bioruptor, Isuru cleaned aliquots of IK31 and IK53 using…

Hybrid capture from degraded DNA: the mysterious case of the vanishing libraries

When we’re working out a protocol or troubleshooting, we spend a lot of time quantifying small quantities of fluids, looking at DNA concentrations on the DeNovix, running tapes…

Polytrichum baits: Cutting mosses

We started our lab work on the Polytrichum hybrid baits project on the 1st of October, by normalising some CTAB-extracted DNA with 0.1X TE to 55 µL of…

Generating a phylogeny of Polytrichum using hybrid baits

The current Next Gen Sequencing lab project at the Botanics involves looking at the phylogeny of Polytrichum section Polytrichum, using hybrid capture. The work will form part of…

Hybrid capture from degraded DNA: bead-cleaning away the adaptor peaks

After testing bead-to-sample ratios of 30:50, 35:50, 40:50, 50:50 and 60:50 on a Thermo Scientific™ GeneRuler™ 50 bp DNA Ladder, using Beckman Coulter AMPure XP beads, we focused…

Hybrid capture from degraded DNA: testing bead cleaning on a 50bp ladder

When we made our Begonia libraries, working with (in some cases) relatively small quantities of very degraded DNA, we should have diluted the adaptors. We didn’t. Consequently, we…

Rare Book Collection

Buchan Hepburn and McDouall East Africa and Angola Album 1904 – 1909

By Helen Bennett, Library & Archives Volunteer New light has been shed on the creation of Logan Garden by a previously unknown album of photographs and watercolours recently…

Rare Book Collection

Interleaving in the RBGE Collections, Part 1: The Flora of Forfarshire

Before publisher’s bindings were de rigueur, texts came in flimsy paper ‘wrappers’, leaving the permanent binding to the new owner. Because of this, many books from the 19th century…

Hybrid capture from degraded DNA: quality metrics for duplicate extractions

For one of the taxa in our study set, Begonia scottii (living collection no. 20170076), we made a few replicate DNA extractions using Qiagen DNeasy plant mini-kits, and…

Meet the Valerian Family

“It’ll stink!  Tell me if you can’t stand it.  My cat adores it.”  Sweaty armpits, musty old rooms, smelly feet were all attempts at describing the smell of…

Hybrid capture from degraded DNA: bead cleaning degraded Begonia DNAs

The set of DNA extractions from one of our test plants (#8, Begonia stictopoda RBGE accession 20170115) contained rather low concentrations of DNA. We decided against preparing NGS…

An expedition to East Ross to rescue the rare Oxytropis halleri

Oxytropis halleri commonly known as Mountain Milk-vetch is a Nationally Rare plant confined only to Scotland.  The species can be found at two inland locations but most populations…

In memory of Gunner Andrew Ewing Calder (1884-1918)

Andrew Ewing Calder was born in Stockbridge, Edinburgh, on the 12th January 1884 to Robert Calder, a blacksmith, and his wife Sarah Jane, who was a tailoress. Calder…

How much liverwort do you need to get 50 μg of DNA?

There’s an exciting project, The 10KP (10,000 Plants) Genome Sequencing Project, that aims to sequence and characterize representative genomes from every major clade of embryophytes, green algae, and…

‘Have you seen the octopus?’

‘Hai visto il polpo?’ *Waves arms to impersonate octopus* ‘Its warm today isn’t it mate’ I said while flapping my arms back to him My current boss called…

Unusual fern Lepisorus thunbergianus

I spotted this unusual fern on a shipment that had come to Vannucci from Japan. The 8-20cm long plants were clinging to the trunk and underside of branches…

Protecting Potatoes: Diversity, Domestication and Darwin

Protecting Potatoes is a new plant display with interpretation for summer 2018 at the Botanics. It can be found in the Demonstration Garden and the Temperate Palm House,…