Author: Laura ForrestPage 4 of 6

Molecular laboratory technician and bryologist, focusing on liverworts and DNA barcoding, with a PhD in Begoniaceae phylogenetics.

Gel electrophoresis of Schistidium ITS DNA

  Once the polymerase chain reaction is over, it’s time to Run The Gel; this is make-or-break time, when we find out if our PCR amplification has actually worked….

Copying moss DNA in the molecular lab

After we extracted a plate’s worth (12 columns by 8 rows, or 96 samples) of Schistidium DNA, the next step in our process is to copy a preselected…

The trials and tribulations of a moss in the lab: DNA extraction

Just over a week into our current Synthesys-funded Schistidium project, and Wolfgang has picked through piles of packets of mosses, selecting the 96 that we would most like…

Campylopus introflexus, an invasive alien on the glasshouse roof

The moss Campylopus introflexus, native to the southern hemisphere, is now considered an invasive plant in parts of Europe and North America. While it occurs on some natural…

Volunteering at the Botanics – bryophytes in our living landscape

There are very few bryophytes growing in the living collections of the Royal Botanic Garden, Edinburgh. What I mean by this is that there are very few bryophytes…

Building on building mosses, a return to Schistidium in the built environment

Monday 27th March was the start of a month-long visit to RBGE by the Fraunhofer Institute for Building Physics‘s Dr Wolfgang Hofbauer, funded by the EU Synthesys Access…

DNA identification of Long’s Long’s Marchantia

Many new species are already included in natural history collections around the world, it’s just that nobody has yet got around to examining the material, recognising that it represents something…

Long’s Marchantia

Formerly the head of our Cryptogam section, and currently an extremely active RBGE Research Associate, David Long is well known and respected for his botanical work in the…

Telaranea murphyae: The non-native endemic that wasn’t

Murphy’s threadwort (Telaranea murphyae) has had a singular position in the British flora. The species was described by renowned bryologist Jean Paton in 1965, from plants collected in…

A phylogeny of Sphaerocarpos

In conjunction with Dr Daniela Schill’s monographic work on Sphaerocarpos, we’ve been building a molecular phylogeny for the genus. We have attempted to extract DNA from 66 accessions,…

Sphaerocarpos, preview to a monograph

The Sphaerocarpales (or “Bottle Liverworts”) form a very distinct group in the complex thalloid liverworts, with ca. 30 species in five genera: originally the group just included Geothallus…

A rapid phylogeny of Marchantia, from the RBGE collections. II. Illuminating our sampling

One of the main problems with sampling largely from herbarium specimens, rather than from material that has been specifically collected for DNA work (rapidly dried in silica gel…

Student projects at RBGE: DNA barcoding of the leafy liverwort genus Herbertus Gray in Europe and a review of the taxonomic status of Herbertus borealis Crundw.

University of Edinburgh/RBGE student David Bell, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2009. Supervisors: Dr David Long and Dr…

The RBGE DNA bank

Over the years, many different people have used the molecular laboratories at RBGE, to work on a multitude of projects on a multitude of plants and fungi. Some…

The RBGE DNA database – how an EDNA number is assigned to a DNA extraction

When people extract DNA in the RBGE molecular lab, we insist that it’s given something we call an EDNA (Edinburgh DNA) number. This links to a database that…

Student projects at RBGE: Barcoding British Liverworts: Metzgeria

University of Edinburgh/RBGE student Kimberley Fackler, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2013. Supervisors: David Bell, Dr David Long…

Student projects at RBGE: Barcoding British Liverworts: Plagiochila (Dumort.) Dumort.

University of Edinburgh/RBGE student Lucy Reed, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2011. Supervisors: Dr David Long, Dr Michelle…

Student projects at RBGE: DNA barcoding British liverworts: Lophocolea

University of Edinburgh Biotechnology student Kenneth McKinlay’s 4th year honours project, 2013. Supervisors: Dr David Long, Dr Laura Forrest Kenneth barcoded all six species of British Lophocolea, L….

A rapid phylogeny of Marchantia, from the RBGE collections. I. Sampling

Not long ago, the only non-crop plant that the mainstream scientific community seemed to be aware of was the brassica Arabidopsis thaliana – easily cultivated, with a short…

Schistidium caps an old wooden fence

Recently in Kufstein, the home of Austrian bryologist Wolfgang Hofbauer, the demolition of an attractive old building and clearing of trees and other plants from the land, leaving…