Tag: BryologyPage 1 of 3

Polytrichum baits: Cutting mosses

We started our lab work on the Polytrichum hybrid baits project on the 1st of October, by normalising some CTAB-extracted DNA with 0.1X TE to 55 µL of…

Generating a phylogeny of Polytrichum using hybrid baits

The current Next Gen Sequencing lab project at the Botanics involves looking at the phylogeny of Polytrichum section Polytrichum, using hybrid capture. The work will form part of…

How much liverwort do you need to get 50 μg of DNA?

There’s an exciting project, The 10KP (10,000 Plants) Genome Sequencing Project, that aims to sequence and characterize representative genomes from every major clade of embryophytes, green algae, and…

Untangling asetate Weissia species in the UK

Dr Des Callaghan spends rather a lot of his time chasing after rare things. He’s an environmental consultant with many strings to his bow, but a particular specialisation…

A violet for Walt

The Shawnee National Forest skirts the midwestern town of Carbondale, which is home to one of the campuses of Southern Illinois University. It’s also one of the prettiest…

A visit to the Californian type locality for the hornwort Phaeoceros proskaueri

One of North America’s endemic hornworts, Phaeoceros proskaueri Stotler, Crand.-Stotl. & W.T.Doyle [also known as Paraphymatoceros proskaueri (Stotler, Crand.-Stotl. & W.T.Doyle) J.C.Villarreal & Cargill] was described from plants collected in the Monterey Bay…

Complex thalloid Asterella lateralis from Panama’s Volcano

During a family holiday to Santiago, Panama in June/July 2011, we snuck in a short bryologising trip, first heading west along the Pan-American Highway, then north, to the…

Snowbird, Utah – Marchantia (Preissia) quadrata from the Rockies

The Botany 2004 meeting was in Snowbird, Utah – a chance to see a different part of the United States (and, of course, to present our research to…

Gyrothyra underwoodiana from Vancouver Island

In April 2004, I flew north from Illinois to met up with a botanical friend, Dr Zoe Badcock. Our meeting point was Vancouver, British Columbia; from there we…

The plants of our lives

There’s something quite melancholy about going back through all the little paper packets of voucher specimens, remembering who and where you were when you collected them, and thinking…

Bryological holiday jobs

The Science building at the Botanics closes down between Christmas and New Year, so any last bits of work for the year have to be packed up and…

Notes on the complex thalloid liverwort genus Plagiochasma

Now that we have six wild-collected accessions of Plagiochasma currently growing on public display in the RBGE Arid House, from China, the US (Texas) and Saudi Arabia, I’ve…

A sudden concentration of Bryologists

Despite a reputation for being rather a rare breed, this week, purely by chance, we have found ourselves with an embarrassment of bryologists at the Gardens. As well…

International Botanical Congress (IBC17) – Living Walls

Our short damp November days offer the perfect opportunity for leafing through reels of photographs from earlier in the year; many of mine are from a short trip…

Barcoding Britain’s Liverworts – progress to date

After RBGE’s initial involvement in land plant DNA barcode marker selection, culminating in a couple of 2009 papers that both utilized bryophyte barcoding data sets, we started a…

What’s the story when there’s no variation?

Enigmatic and isolated although it is, it seems that our Australian colleagues have now “got their eye in” for complex thalloid liverwort Monocarpus sphaerocarpus – after many years…

Losing the story with a moss from Panama City

Spring Break’s a big thing in the US, and spring of 2005, Juan Carlos Villarreal and I spent ours on a road-trip down through Louisianna, looking for the…

Panamanian mosses from the back of the freezer

Several years back, I postdocced in Barbara Crandall-Stotler’s lab in Southern Illinois University, Carbondale. In the late Autumn of 2003, Panamanian bryologist Noris Salazar Allen spent a few…

Cleaning the Schistidium PCRs

Once we realised that most of our plate of Schistidium ITS2 amplifications had been successful, it was an easy decision to process them all for DNA sequencing. If…

Gel electrophoresis of Schistidium ITS DNA

  Once the polymerase chain reaction is over, it’s time to Run The Gel; this is make-or-break time, when we find out if our PCR amplification has actually worked….