Tag: Synthesys

DNA Sequencing Natural History Specimens Using New Sequencing Platforms and Protocols: a 1-day meeting at RBGE 11/07/2017

Rapid developments in high-throughput sequencing platforms are providing a step change in the recoverability of DNA sequence data from natural history collections. Short-read massively parallel sequencers are intrinsically…

Testing extractions – comparing DNA on agarose gels

Looking at the capture plates from the two DNA extraction protocols that were tested on our QIAcube, it was fairly obvious that a lot more plant fragments and…

Describing your DNA

One of the amazing things about the polymerase chain reaction, PCR, is how little starting DNA is needed, with an exponential increase in the number of copies of…

Letting the robot do its job

Having got together two plates of tubes with little bits of plant and lichen tissue in them, and pulverised them with tungsten beads in a TissueLyser for a…

Bits of bamboo

In the Herbarium at RBGE, we store a huge number of sheets of archival quality paper with squashed and dried plant specimens stuck to them. These have been…

Dealing with DNA extraction protocol changes

It’s a horrible and unwelcome upheaval to have to change a protocol that works, but that’s the situation in which we have found ourselves with our semi-robotic DNA…

Cleaning the Schistidium PCRs

Once we realised that most of our plate of Schistidium ITS2 amplifications had been successful, it was an easy decision to process them all for DNA sequencing. If…

Campylopus introflexus, an invasive alien on the glasshouse roof

The moss Campylopus introflexus, native to the southern hemisphere, is now considered an invasive plant in parts of Europe and North America. While it occurs on some natural…

Volunteering at the Botanics – bryophytes in our living landscape

There are very few bryophytes growing in the living collections of the Royal Botanic Garden, Edinburgh. What I mean by this is that there are very few bryophytes…

Building on building mosses, a return to Schistidium in the built environment

Monday 27th March was the start of a month-long visit to RBGE by the Fraunhofer Institute for Building Physics‘s Dr Wolfgang Hofbauer, funded by the EU Synthesys Access…