Category: SciencePage 29 of 36

Latest science blog posts from the Royal Botanic Garden Edinburgh

First Audio Leaflet: Dawyck Scottish Trees Trail

Apps Apps Apps For several years now we have been looking for a way to make appropriate use of mobile phones to deliver interpretation material. Smart phones really…

Capturing Genes from Herbaria. IX. Hybrid capture

By mid-May 2015, we had 32 separate Inga umbellifera libraries, 15 generated using the Illumina Tru-Seq Nano library preparation kits, and 17 with the NEBNext Ultra library preparation…

Finding Monocarpus, in the field

Sadly, although not surprisingly, I was not able to amplify the regions of Monocarpus DNA needed to compare it to other complex thalloid liverworts from a 1950s collection that we had…

Where the Tropics meet the Arctic – Scotland

Some of the most remote and beautiful wilderness landscapes in Scotland are in the extreme north-west, in Sutherland, so-called from the Vikings who regarded it as the south…

John Hutton Balfour Excursions

“Excursions may be truly said to be the life of the botanist. They enable him to study the science practically, by the examination of plants in their living state, and…

Biography of John Hutton Balfour

John Hutton Balfour Born: 15 September 1808 Place of Birth: Buccleuch Place, Edinburgh Died: 11 February 1884 Place of Death: Inverleith House, Edinburgh Occupation: Botanist and Teacher The…

Finding Monocarpus, in the herbarium

At the Royal Botanic Garden Edinburgh we’ve been working on the phylogeny of the complex thalloid liverworts for rather a while now. David Long presented a poster on…

On Monocarpus

As far as liverworts go, Monocarpus is a rather strange plant. It’s very small, in itself not that unusual for a bryophyte, but rather problematic if you need…

Dry tropical forest movie making

  Seasonally Dry Tropical Forests are found throughout Latin America from Mexico to south-western Brazil and northern Argentina. Dry forest is the world’s most threatened tropical forest due to…

300,000 specimens imaged and online!

Yesterday saw the 300,000th specimen from our herbarium collection imaged and made available on our website. This is a big milestone for the digitisation programme, which has been…

Wartime Rhododendron

  In the Autumn of 1914, George Forrest was travelling in China. His letters written at the time mention the difficulties he was facing in getting permission to…

Capturing Genes from Herbaria. VIII. Amplification

Both the Tru-Seq and NEB libraries were amplified pre-hybrid capture – another step at which modifications were made, according to how much DNA there was in each library….

Capturing Genes from Herbaria. VII. Comparisons.

As previously mentioned, we tested two different kits in our NBAF project. The first is the Illumina Tru-Seq Nano library preparation kit (FC-121-4001), which recommends a starting DNA…

Amorphophallus titanum – Preserving it for posterity

The flowering of our Amorphophallus titanum (titan arum) was a tremendous event with c 19,000 people visiting the Glasshouses to see the plant growing from a small bud…

Capturing Genes from Herbaria. VI. Size selection

A few days ago, I read a tweet from the Botany2015 meeting in Alberta that described DNA extracted from herbarium specimens as “pre-sheared”. This resonates with our own…

British Algae online

Here at the RBGE we have just finished digitising our collection of British Rhodophyta (Red Algae), a total of 7850 specimens. Our collection dates from present day right…

Capturing Genes from Herbaria. V. Fragmenting the DNA

The mantra for many years for next generation sequencing has been, like “garbage in, garbage out”, that the optimal starting point is high quality, high molecular weight DNA….

Capturing Genes from Herbaria. IV. DNA

In March this year, having already chosen and obtained the plant material that we were going to use for our NBAF project on using a hybrid bait protocol…

Capturing Genes from Herbaria. III. The Samples.

Having chosen Inga umbellifera as the study organism for our NBAF-funded project to test the use of hybrid baits for recovery of DNA sequences from herbarium material, we…