Tag: hybrid capture
Following on from the rather unpredictable results we obtained from fragmenting duplicate aliquots of CTAB-extracted Polytrichum DNA in the Bioruptor, Isuru cleaned aliquots of IK31 and IK53 using…
We started our lab work on the Polytrichum hybrid baits project on the 1st of October, by normalising some CTAB-extracted DNA with 0.1X TE to 55 µL of…
The current Next Gen Sequencing lab project at the Botanics involves looking at the phylogeny of Polytrichum section Polytrichum, using hybrid capture. Polytrichum commune, photographed by David Bell…
For one of the taxa in our study set, Begonia scottii (living collection no. 20170076), we made a few replicate DNA extractions using Qiagen DNeasy plant mini-kits, and…
The set of DNA extractions from one of our test plants (#8, Begonia stictopoda RBGE accession 20170115) contained rather low concentrations of DNA. We decided against preparing NGS…
Keen to see the effects of different specimen preservation techniques on DNA quantity and quality, we have assessed extractions of DNA from nine Begonia accessions x seven preservation…
In order to look at the effects of herbarium preservation methods on DNA quality, Hannah Wilson and Mark Hughes took a trip down to our research glasshouses, and…
The megadiverse genus Begonia L. is one of the world’s largest plant genera, comprising over 1,800 species, an estimated 200 of which are endemic to New Guinea. A…
When working out new protocols, it’s very useful to have a big stash of DNA to test them on. Just now, in a collaborative project with Dr Michelle…
As previously mentioned, we tested two different kits in our NBAF project. The first is the Illumina Tru-Seq Nano library preparation kit (FC-121-4001), which recommends a starting DNA…
A few days ago, I read a tweet from the Botany2015 meeting in Alberta that described DNA extracted from herbarium specimens as “pre-sheared”. This resonates with our own…