Tag: DNA barcoding

Barcoding Britain’s Liverworts – progress to date

After RBGE’s initial involvement in land plant DNA barcode marker selection, culminating in a couple of 2009 papers that both utilized bryophyte barcoding data sets, we started a…

What’s the story when there’s no variation?

Enigmatic and isolated although it is, it seems that our Australian colleagues have now “got their eye in” for complex thalloid liverwort Monocarpus sphaerocarpus – after many years…

Only 12 Seconds To Poop – But Enough Information To Understand An Ecosystem

It may only take a mammal 12 seconds to poop – but poo contains a treasure trove of information about the animal and its environment that can take…

Cleaning the Schistidium PCRs

Once we realised that most of our plate of Schistidium ITS2 amplifications had been successful, it was an easy decision to process them all for DNA sequencing. If…

Gel electrophoresis of Schistidium ITS DNA

  Once the polymerase chain reaction is over, it’s time to Run The Gel; this is make-or-break time, when we find out if our PCR amplification has actually worked….

Copying moss DNA in the molecular lab

After we extracted a plate’s worth (12 columns by 8 rows, or 96 samples) of Schistidium DNA, the next step in our process is to copy a preselected…

The trials and tribulations of a moss in the lab: DNA extraction

Just over a week into our current Synthesys-funded Schistidium project, and Wolfgang has picked through piles of packets of mosses, selecting the 96 that we would most like…

Building on building mosses, a return to Schistidium in the built environment

Monday 27th March was the start of a month-long visit to RBGE by the Fraunhofer Institute for Building Physics‘s Dr Wolfgang Hofbauer, funded by the EU Synthesys Access…

DNA identification of Long’s Long’s Marchantia

Many new species are already included in natural history collections around the world, it’s just that nobody has yet got around to examining the material, recognising that it represents something…

A phylogeny of Sphaerocarpos

In conjunction with Dr Daniela Schill’s monographic work on Sphaerocarpos, we’ve been building a molecular phylogeny for the genus. We have attempted to extract DNA from 66 accessions,…

Sphaerocarpos, preview to a monograph

The Sphaerocarpales (or “Bottle Liverworts”) form a very distinct group in the complex thalloid liverworts, with ca. 30 species in five genera: originally the group just included Geothallus…

A rapid phylogeny of Marchantia, from the RBGE collections. II. Illuminating our sampling

One of the main problems with sampling largely from herbarium specimens, rather than from material that has been specifically collected for DNA work (rapidly dried in silica gel…

Student projects at RBGE: DNA barcoding of the leafy liverwort genus Herbertus Gray in Europe and a review of the taxonomic status of Herbertus borealis Crundw.

University of Edinburgh/RBGE student David Bell, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2009. Supervisors: Dr David Long and Dr…

The RBGE DNA bank

Over the years, many different people have used the molecular laboratories at RBGE, to work on a multitude of projects on a multitude of plants and fungi. Some…

Student projects at RBGE: Barcoding British Liverworts: Metzgeria

University of Edinburgh/RBGE student Kimberley Fackler, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2013. Supervisors: David Bell, Dr David Long…

Student projects at RBGE: Barcoding British Liverworts: Plagiochila (Dumort.) Dumort.

University of Edinburgh/RBGE student Lucy Reed, studying for the Masters degree in the Biodiversity and Taxonomy of Plants; thesis submitted August 2011. Supervisors: Dr David Long, Dr Michelle…

Student projects at RBGE: DNA barcoding British liverworts: Lophocolea

University of Edinburgh Biotechnology student Kenneth McKinlay’s 4th year honours project, 2013. Supervisors: Dr David Long, Dr Laura Forrest Kenneth barcoded all six species of British Lophocolea, L….

In plain sight – the mosses that grow on British walls

Plant diversity does not have to be far-flung and exotic to be worth studying; even within Scotland, there are unanswered questions about plant distributions. Growing in our towns and…

A mixed message on PCR additives in Aneura

This last week I’ve actually managed to spend a bit of time in the lab, trying to get some gaps filled in a DNA barcoding matrix for simple…

Hidden diversity in unexpected places – moss growth on modern building surfaces

Back in 2014, staff in the molecular lab and herbarium at RBGE greatly enjoyed a three-week visit from Austrian Dr Wolfgang Hofbauer. With funding from the EU SYNTHESYS…