Tag: DNAPage 2 of 3
It may only take a mammal 12 seconds to poop – but poo contains a treasure trove of information about the animal and its environment that can take…
Murphy’s threadwort (Telaranea murphyae) has had a singular position in the British flora. The species was described by renowned bryologist Jean Paton in 1965, from plants collected in…
The Sphaerocarpales (or “Bottle Liverworts”) form a very distinct group in the complex thalloid liverworts, with ca. 30 species in five genera: originally the group just included Geothallus…
Over the years, many different people have used the molecular laboratories at RBGE, to work on a multitude of projects on a multitude of plants and fungi. Some…
When people extract DNA in the RBGE molecular lab, we insist that it’s given something we call an EDNA (Edinburgh DNA) number. This links to a database that…
What do Giant panda eat? The answer might seem obvious but the reality is far from simplistic. The diet of the Giant panda (Ailuropoda melanoleuca) is highly specialised…
This last week I’ve actually managed to spend a bit of time in the lab, trying to get some gaps filled in a DNA barcoding matrix for simple…
The relative structural simplicity of some groups of mosses can disguise their uniqueness, especially when simplified features have evolved multiple times within the same family from ancestors with…
One of the earliest plastid genomes to be sequenced, in the late 1980s (Ohyama et al.), was that of Marchantia polymorpha, one of the commonest liverworts around town,…
By mid-May 2015, we had 32 separate Inga umbellifera libraries, 15 generated using the Illumina Tru-Seq Nano library preparation kits, and 17 with the NEBNext Ultra library preparation…
At the Royal Botanic Garden Edinburgh we’ve been working on the phylogeny of the complex thalloid liverworts for rather a while now. David Long presented a poster on…
Both the Tru-Seq and NEB libraries were amplified pre-hybrid capture – another step at which modifications were made, according to how much DNA there was in each library….
As previously mentioned, we tested two different kits in our NBAF project. The first is the Illumina Tru-Seq Nano library preparation kit (FC-121-4001), which recommends a starting DNA…
A few days ago, I read a tweet from the Botany2015 meeting in Alberta that described DNA extracted from herbarium specimens as “pre-sheared”. This resonates with our own…
The mantra for many years for next generation sequencing has been, like “garbage in, garbage out”, that the optimal starting point is high quality, high molecular weight DNA….
In March this year, having already chosen and obtained the plant material that we were going to use for our NBAF project on using a hybrid bait protocol…
Having chosen Inga umbellifera as the study organism for our NBAF-funded project to test the use of hybrid baits for recovery of DNA sequences from herbarium material, we…
About 300 species of Inga (Leguminosae: Mimosoideae: Ingeae) grow in lowland and montane rain forest throughout the humid tropical zone, from Mexico to Uruguay. Most species diversity is…