Tag: degraded DNA
Following on from the rather unpredictable results we obtained from fragmenting duplicate aliquots of CTAB-extracted Polytrichum DNA in the Bioruptor, Isuru cleaned aliquots of IK31 and IK53 using…
When we’re working out a protocol or troubleshooting, we spend a lot of time quantifying small quantities of fluids, looking at DNA concentrations on the DeNovix, running tapes…
After testing bead-to-sample ratios of 30:50, 35:50, 40:50, 50:50 and 60:50 on a Thermo Scientific™ GeneRuler™ 50 bp DNA Ladder, using Beckman Coulter AMPure XP beads, we focused…
When we made our Begonia libraries, working with (in some cases) relatively small quantities of very degraded DNA, we should have diluted the adaptors. We didn’t. Consequently, we…
For one of the taxa in our study set, Begonia scottii (living collection no. 20170076), we made a few replicate DNA extractions using Qiagen DNeasy plant mini-kits, and…
The set of DNA extractions from one of our test plants (#8, Begonia stictopoda RBGE accession 20170115) contained rather low concentrations of DNA. We decided against preparing NGS…
Quality and quantity of DNA recovered from stored plant material is becoming more important as DNA sequencing technologies change from the relatively simple Sanger sequencing, to next generation…
Keen to see the effects of different specimen preservation techniques on DNA quantity and quality, we have assessed extractions of DNA from nine Begonia accessions x seven preservation…
In order to look at the effects of herbarium preservation methods on DNA quality, Hannah Wilson and Mark Hughes took a trip down to our research glasshouses, and…
The megadiverse genus Begonia L. is one of the world’s largest plant genera, comprising over 1,800 species, an estimated 200 of which are endemic to New Guinea. A…
Rapid developments in high-throughput sequencing platforms are providing a step change in the recoverability of DNA sequence data from natural history collections. Short-read massively parallel sequencers are intrinsically…
Looking at the capture plates from the two DNA extraction protocols that were tested on our QIAcube, it was fairly obvious that a lot more plant fragments and…
One of the amazing things about the polymerase chain reaction, PCR, is how little starting DNA is needed, with an exponential increase in the number of copies of…
Having got together two plates of tubes with little bits of plant and lichen tissue in them, and pulverised them with tungsten beads in a TissueLyser for a…
In the Herbarium at RBGE, we store a huge number of sheets of archival quality paper with squashed and dried plant specimens stuck to them. These have been…
It’s a horrible and unwelcome upheaval to have to change a protocol that works, but that’s the situation in which we have found ourselves with our semi-robotic DNA…
It’s a confusing world out there – betaine, DMSO, bovine serum albumin (BSA), trehalose, glycerol, formamide – the list of things that you can throw into a PCR…